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43-531 (0.3ug/ml) staining of Daudi (A), Jurkat (B) and K562 (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-531 (5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-531 (0.3ug/ml) staining of Daudi (A), Jurkat (B) and K562 (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

OAS2 Antibody

CATALOG NUMBER: 43-531

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: QPNKCFLEQIDSAVN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell lines Daudi, Jurkat and K562 (calculated MW of 78.8kDa according to NP_002526.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lin et al, J Immunol. 2009 Dec 15;183(12):8035-43. PMID: 19923450). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize reported isoform 1 (NP_058197.2) and isoform 2 (NP_002526.2).
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
OAS2
Additional Names:
OAS2, 2'-5'-oligoadenylate synthetase 2, 69/71kDa, (2'-5')oligo(A) synthetase 2, 2'-5'-oligoadenylate synthase 2, 2-5A synthase 2, p69 OAS / p71 OAS
Protein Accession Number:
NP_058197.2,NP_002526.2
Protein Gi Number:
74229019
Ncbi Gene Id Number:
4939
Background Reference 1:
Dugan JW, Albor A, David L, Fowlkes J, Blackledge MT, Martin TM, Planck SR, Rosenzweig HL, Rosenbaum JT, Davey MP.Nucleotide oligomerization domain-2 interacts with 2'-5'-oligoadenylate synthetase type 2 and enhances RNase-L function in THP-1 cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-531

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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