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Western blot analysis of OCIAD1 in 293 cell lysate with OCIAD1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of OCIAD1 in 293 cells with OCIAD1 antibody at 20 μg/mL.
Western blot analysis of OCIAD1 in 293 cell lysate with OCIAD1 antibody at (A) 1 and (B) 2 μg/mL.
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OCIAD1 Antibody

CATALOG NUMBER: 5461

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Mouse: (100%), Rat: (94%)
Immunogen:
OCIAD1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human OCIAD1.
The immunogen is located within the last 50 amino acids of OCIAD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
OCIAD1 antibody can be used for detection of OCIAD1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Purification:
OCIAD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
OCIAD1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
OCIAD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OCIAD1
Additional Names:
OCIAD1 Antibody: OCIA, ASRIJ, TPA018, OCIA, OCIA domain-containing protein 1, Ovarian carcinoma immunoreactive antigen
Protein Accession Number:
NP_060300
Protein Gi Number:
8923427
Ncbi Gene Id Number:
54940
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OCIAD1 Antibody: OCIAD1 was identified via immunoscreening of an ovarian carcinoma cDNA library from ovarian cancer patients and is expressed in multiple tissues including ovary, placenta, brain, testis, prostate, and mammary gland. Two isoforms of OCIAD1 are known to exist; the shorter isoform is restricted to the central nervous system. OCIAD1 is a transmembrane protein whose overexpression in HEY ovarian cancer cells increased lysophosphatidic acid- (LPA-)induced, but not basal level cell adhesion to extracellular matrix proteins collagen I and laminin10/11. This adhesion is not blocked by LY294002 and GF109203X, suggesting that OCIAD1 does not use protein kinase C and PI3 kinase signaling pathways to exert its effect on adhesion.
Background Reference 1:
Luo LY, Soosaipillai A, and Diamandis EP. Molecular cloning of a novel human gene on chromosome 4p11 by immunoscreening of an ovarian carcinoma cDNA library. Biochem. Biophys. Res. Commun.2001; 280:401-6.
Background Reference 2:
Sengupta S, Michener CM, Escobar P, et al. Ovarian cancer immuno-reactive antigen domain containing 1 (OCIAD1), a key player in ovarian cancer cell adhesion. Gynecologic Oncol.2008; 109:226-33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5461

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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