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46-880 (10ug/ml) staining of nuclei HeLa cells (green). Detected by immunofluorescence.
46-880 (1.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-880 (10ug/ml) staining of nuclei HeLa cells (green). Detected by immunofluorescence.

ODZ3 Antibody

CATALOG NUMBER: 46-880

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NNPASKIHDVKDY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Human Brain lysates and in lysates of cell line NIH 3T3 gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Brain (Cerebellum) shows nuclear staining of the granular cell layer. Recommended concentration, 1.5-3ug/ml.Immunofluorescence: Nuclear staining (green) in HeLa cells.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ODZ3
Additional Names:
ODZ3, odz, odd Oz/ten-m homolog 3 (Drosophila), FLJ10474, FLJ10886, KIAA1455, TNM3, Ten-m3, ODZ3-like protein, odd Oz/Ten-m homolog 3, protein Odd Oz/ten-m homolog 3, ten-3, tenascin-M3, teneurin-3
Protein Accession Number:
NP_001073946.1
Protein Gi Number:
122937400
Ncbi Gene Id Number:
55714
Background Reference 1:
Ben-Zur T, Feige E, Motro B, Wides R. The mammalian Odz gene family: homologs of a Drosophila pair-rule gene withexpression implying distinct yet overlapping developmental roles. Dev Biol. 2000 Jan 1;217(1):107-20.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-880

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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