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Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.
Immunofluorescence of OGFOD1 in Daudi cells with OGFOD1 antibody at 20 μg/mL.
Western blot analysis of OGFOD1 in Daudi cell lysate with OGFOD1 antibody at 1 μg/mL.
Trial Size

OGFOD1 Antibody

CATALOG NUMBER: 6177

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
OGFOD1 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human OGFOD1.
The immunogen is located within the first 50 amino acids of OGFOD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
OGFOD1 antibody can be used for detection of OGFOD1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
OGFOD1 antibody is predicted to not cross-react with other TPA1 protein family members. At least two isoforms are known to exist; this antibody will only detect the larger isform.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
OGFOD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
OGFOD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
OGFOD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OGFOD1
Additional Names:
OGFOD1 Antibody: TPA1, KIAA1612, TPA1, 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1, Termination and polyadenylation 1 homolog
Protein Accession Number:
NP_060703
Protein Gi Number:
94536836
Ncbi Gene Id Number:
55239
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OGFOD1 Antibody: OGFOD1, a member of the 2-OG-Fe(II) dioxygenase family, plays an important role in ischemic survival and stress. It is an oxidoreductase containing a Prolyl 4-hydroxylase alpha subunit. An OGFOD1 iron binding residue is required for ATPAF1 gene expression. As a novel stress granule component, OGFOD1 plays important proapoptotic roles in the regulation of translation and HRI-mediated phosphorylation of eIF2alpha in cells.
Background Reference 1:
Saito K, Adachi N, Koyama H et al. OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling. FEBS Lett. 2010; 584:3340-7.
Background Reference 2:
Wehner KA, Schutz S and Sarnow P. OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress. Mol. Cell. Biol. 2010; 30:2006-16.
Background Reference 3:
Marina O, Hainz U, Biernacki MA, et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res. 2010; 70:1344-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6177

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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