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Western blot analysis of OGFOD2 in 293 cell lysate with OGFOD2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of OGFOD2 in 293 cell lysate with OGFOD2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

OGFOD2 Antibody

CATALOG NUMBER: 6179

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
OGFOD2 antibody was raised against a 17 amino acid synthetic peptide near the center of human OGFOD2.
The immunogen is located within amino acids 140 - 190 of OGFOD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
OGFOD2 antibody can be used for detection of OGFOD2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
OGFOD2 antibody is predicted to not cross-react with other TPA1 protein family members. At least three isoforms are known to exist; this antibody will only detect the two larger isoforms.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Purification:
OGFOD2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
OGFOD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
OGFOD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OGFOD2
Additional Names:
OGFOD2 Antibody: 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2
Protein Accession Number:
NP_078899
Protein Gi Number:
13375846
Ncbi Gene Id Number:
79676
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OGFOD2 Antibody: OGFOD2 is a member of the 2-OG-Fe(II) dioxygenase family which plays an important role in ischemic survival and stress. Like the related protein OGFOD1, OGFOD2 is an oxidoreductase containing a Prolyl 4-hydroxylase alpha subunit. Little is known about the function of OGFOD2, but its similarity to OGFOD1 suggests that it is a novel stress granule component that may play an important proapoptotic role in the regulation of translation and HRI-mediated phosphorylation of eIF2alpha in cells.
Background Reference 1:
Saito K, Adachi N, Koyama H et al. OGFOD1, a member of the 2-oxoglutarate and iron dependent dioxygenase family, functions in ischemic signaling. FEBS Lett. 2010; 584:3340-7.
Background Reference 2:
Wehner KA, Schutz S and Sarnow P. OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress. Mol. Cell. Biol. 2010; 30:2006-16.
Background Reference 3:
Marina O, Hainz U, Biernacki MA, et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res. 2010; 70:1344-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6179

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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