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Western blot analysis of extracts of various cell lines, using OGFR antibody (22-678) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using OGFR antibody (22-678) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using OGFR antibody (22-678). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using OGFR antibody (22-678) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

OGFR Antibody

CATALOG NUMBER: 22-678

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human OGFR (NP_031372.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Positive Control 1:
A-431
Positive Control 2:
Mouse kidney
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
OGFR
Additional Names:
OGFR, opioid growth factor receptor, HGNC:15768, 7-60 protein, zeta-type opioid receptor, met-enkephalin receptor,
Ncbi Gene Id Number:
11054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a receptor for opioid growth factor (OGF), also known as [Met(5)]-enkephalin. OGF is a negative regulator of cell proliferation and tissue organization in a variety of processes. The encoded unbound receptor for OGF has been localized to the outer nuclear envelope, where it binds OGF and is translocated into the nucleus. The coding sequence of this gene contains a polymorphic region of 60 nt tandem imperfect repeat units. Several transcripts containing between zero and eight repeat units have been reported.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-678

List Size:
100 uL

List Price:

$436.00

Datasheet
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