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Western blot analysis of OGT antibody in mouse bladder tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with OGT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of OGT antibody in mouse bladder tissue lysates (35ug/lane)

OGT Antibody

CATALOG NUMBER: 62-409

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Pig, Rabbit, Rat
Immunogen:
This OGT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1017-1046 amino acids from the C-terminal region of human OGT.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
117 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OGT
Additional Names:
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT, OGT
Protein Accession Number:
O15294
Ncbi Gene Id Number:
8473
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal.
Background Reference 1:
Roeder,R.G., Nature 459 (7245), 455-459 (2009)
Background Reference 2:
Taylor,R.P., J. Biol. Chem. 284 (6), 3425-3432 (2009)
Background Reference 3:
Slawson,C., Mol. Biol. Cell 19 (10), 4130-4140 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-409

List Size:
400 ul

List Price:

$495.00

Datasheet
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