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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Olfm1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

Olfm1 Antibody

CATALOG NUMBER: 61-447

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Chicken
Immunogen:
This Olfm1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the N-terminal region of human Olfm1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
55 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OLFM1
Additional Names:
Noelin, Neuronal olfactomedin-related ER localized protein, Olfactomedin-1, OLFM1, NOE1, NOEL1
Protein Accession Number:
Q99784
Protein Gi Number:
206729935
Ncbi Gene Id Number:
10439
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Olfm1 shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of this protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue.
Background Reference 1:
Yokoyama M.,DNA Res. 3:311-320(1996).
Background Reference 2:
Yu W., Genome Res. 7:353-358(1997).
Background Reference 3:
Liu T.,J. Proteome Res. 4:2070-2080(2005).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-447

List Size:
400 ul

List Price:

$495.00

Datasheet
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