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Western blot analysis of OLIG1 in A549 cell lysate with OLIG1 antibody at 1 μg/ml.
Immunohistochemistry of OLIG1 in human brain tissue with OLIG1 antibody at 5 μg/mL.
Immunofluorescence of OLIG1 in human brain tissue with OLIG1 antibody at 20 μg/mL.
Western blot analysis of OLIG1 in A549 cell lysate with OLIG1 antibody at 1 μg/ml.
Trial Size

OLIG1 Antibody

CATALOG NUMBER: 7741

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (83%), Mouse: (78%)
Immunogen:
OLIG1 antibody was raised against an 18 amino acid peptide near the amino terminus of human OLIG1.
The immunogen is located within amino acids 50 - 100 of OLIG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
OLIG1 antibody can be used for detection of OLIG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
OLIG1 antibody is human specific. It is predicted to not cross-react with other members of the OLIG family of proteins.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 30 kDa
Observed: 29 kDa
Purification:
OLIG1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
OLIG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
OLIG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
OLIG1
Additional Names:
OLIG1 Antibody: BHLHB6, BHLHE21, BHLHB6, Oligodendrocyte transcription factor 1, Class B basic helix-loop-helix protein 6, Oligo1
Protein Accession Number:
NP_620450
Protein Gi Number:
237757328
Ncbi Gene Id Number:
116448
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG1 is first expressed in the dorsal portion of the p3 progenitor domain of the ventral neural tube while OLIG2 is first observed in the ventral most p3 domain (2). OLIG1 has been shown to be a SMAD cofactor involved in cell motility induced by transforming growth factor-beta (TGF-beta) (3).
Background Reference 1:
Zhou Q, Wang S, and Anderson DJ. Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 2000; 25:331-43.
Background Reference 2:
Lu QR, Yuk D, Alberta JA, et al. Sonic Hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron 2000; 25:317-29.
Background Reference 3:
Motizuki M, Isogaya K, Miyake K, et al. Oligodendrocyte transcription factor 1 (Olig1) is a Smad cofactor involved in cell motility induced by transforming growth factor-b. J. Biol. Chem. 2013; 288:18911-22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7741

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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