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Western blot analysis of OLIG3 Antibody in mouse spleen tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue with OLIG3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of OLIG3 Antibody in mouse spleen tissue lysates (35ug/lane)

OLIG3 Antibody

CATALOG NUMBER: 63-558

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This OLIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the Central region of human OLIG3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OLIG3
Additional Names:
Oligodendrocyte transcription factor 3, Oligo3, Class B basic helix-loop-helix protein 7, bHLHb7, Class E basic helix-loop-helix protein 20, bHLHe20, OLIG3, BHLHB7, BHLHE20
Protein Accession Number:
Q7RTU3
Protein Gi Number:
51701664
Ncbi Gene Id Number:
167826
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Background Reference 1:
Muller,T., et.al., Genes Dev. 19 (6), 733-743 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-558

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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