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Antibody used in IHC on Human Brain, cerebellum at 5.0 ug/ml.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on Human Brain, cerebellum at 5.0 ug/ml.

ONECUT1 Antibody

CATALOG NUMBER: 25-436

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ONECUT1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ONECUT1 antibody can be used for detection of ONECUT1 by ELISA at 1:312500. ONECUT1 antibody can be used for detection of ONECUT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
51 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ONECUT1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ONECUT1
Additional Names:
ONECUT1, HNF-6, HNF6, HNF6A
Protein Accession Number:
NP_004489
Protein Gi Number:
24307887
Ncbi Gene Id Number:
3175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ONECUT1 belongs to the CUT homeobox family. It contains 1 CUT DNA-binding domain and 1 homeobox DNA-binding domain. It is a transcriptional activator. ONECUT1 binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. It is important for liver genes transcription.
Background Reference 1:
Jiang, X., (2008) Oncol. Rep. 19 (1), 157-163.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-436

List Size:
100 ul

List Price:

$519.00

Datasheet
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