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Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.

ONECUT3 Antibody

CATALOG NUMBER: 59-624

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ONECUT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-419 amino acids from the C-terminal region of human ONECUT3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ONECUT3
Additional Names:
One cut domain family member 3, One cut homeobox 3, Transcription factor ONECUT-3, OC-3, ONECUT3
Protein Accession Number:
O60422
Ncbi Gene Id Number:
390874
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ONECUT3 is transcriptional activator. It binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).
Background Reference 1:
Grimwood, J., et al. Nature 428(6982):529-535(2004)
Background Reference 2:
Vanhorenbeeck, V., et al. Biochem. Biophys. Res. Commun. 292(4):848-854(2002)
Background Reference 3:
Venter, J.C., et al. Science 291(5507):1304-1351(2001)
Background Reference 4:
Jacquemin, P., et al. J. Biol. Chem. 274(5):2665-2671(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-624

List Size:
400 ul

List Price:

$495.00

Datasheet
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