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Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of ORAI1 in human ovary tissue with ORAI1 antibody at 2.5 μg/ml.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.
Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

ORAI1 Antibody

CATALOG NUMBER: 4281

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (93%), Chicken: (100%)
Immunogen:
ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
The immunogen is located within the last 50 amino acids of ORAI1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 - 2 μg/mL. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Positive Control 1:
Cat. No. 1316 - Human Ovary Tissue Lysate
Positive Control 2:
Cat. No. 11-201 - Human Ovary Tissue Slide
Positive Control 3:
Cat. No. 10-901 - Human Spleen Tissue Slide
Purification:
ORAI1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ORAI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ORAI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ORAI1
Additional Names:
ORAI1 Antibody: IMD9, ORAT1, CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1
Protein Accession Number:
Q96D31
Protein Gi Number:
97180269
Ncbi Gene Id Number:
84876
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ORAI1 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.
Background Reference 1:
Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol.2001; 19:497-521.
Background Reference 2:
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature2006; 441:179-85.
Background Reference 3:
Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta.2006; 1763:1161-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4281

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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