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46-765 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-765 (0.1ug/ml) staining of Human Skin lysate (35ug protein in RIPA buffer). . Detected by chemiluminescence.
46-765 (0.03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ORAI1 Antibody

CATALOG NUMBER: 46-765

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-KKQPGQPRPTSKP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 50-55kDa band observed in Human Placenta and Skin lysates (calculated MW of 32.7kDa according to NP_116179.2). The observed molecular weight corresponds to different antibodies from other commercial sources and with earlier findings in literature (Li et al,; PMID: 17702753). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ORAI1
Additional Names:
ORAI1, CRACM1, ORAI calcium release-activated calcium modulator 1, FLJ14466, ORAT1, TMEM142A, calcium release-activated calcium channel protein 1, calcium release-activated calcium modulator 1, transmembrane protein 142A
Protein Accession Number:
NP_116179.2
Protein Gi Number:
38016943
Ncbi Gene Id Number:
84876
Background Reference 1:
Vig M, DeHaven WI, Bird GS, Billingsley JM, Wang H, Rao PE, Hutchings AB, Jouvin MH, Putney JW, Kinet JP. Defective mast cell effector functions in mice lacking the CRACM1 pore subunit of store-operated calcium release-activated calcium channels. Nat Immunol. 2008 Jan;9(1):89-96. Epub 2007 Dec 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-765

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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