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Western blot analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 5 μg/mL.
Immunofluorescence of ORAI1 in Human Spleen tissue with ORAI1 antibody at 20 ug/mL.
Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 2.5 μg/mL.
Western blot analysis of ORAI1 in human spleen tissue lysate with ORAI1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

ORAI1 Antibody [6D11A11]

CATALOG NUMBER: PM-5207

Clonality:
Monoclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
ORAI1 antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Positive Control 1:
Cat. No. 1366 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 33 kDa
Observed: 50 kDa
Purification:
ORAI1 Monoclonal Antibody is Protein A purified.
Clonality:
Monoclonal
Isotype:
IgG2b
physical-state:
Liquid
Buffer:
ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ORAI1 monoclonal antibody can be stored at -20°C, stable for one year.
Ncbi Official Symbol:
ORAI1
Additional Names:
ORAI1 Antibody [6D11A11] : IMD9, ORAT1, CRACM1, TMEM142A
Protein Accession Number:
Q96D31
Protein Gi Number:
97180269
Ncbi Gene Id Number:
84876
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.
Background Reference 1:
Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
Background Reference 2:
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
Background Reference 3:
Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-5207

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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