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46-101 (2ug/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.
46-101 (3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-101 (2ug/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by chemiluminescence.

ORC3L Antibody

CATALOG NUMBER: 46-101

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: QKTDHVARLTWGGC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and HeLa (calculated MW of 82.3kDa according to NP_862820.1 and NP_036513.2). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2).
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ORC3L
Additional Names:
ORC3L, origin recognition complex, subunit 3-like (yeast), LAT, ORC3, LATHEO, IMAGE50150, origin recognition complex, subunit 3 (yeast homolog)-like, OTTHUMP00000040611, homolog of latheo, Drosophila, RP3-486L4.1, origin recognition complex, subunit 3
Protein Accession Number:
NP_862820.1,NP_036513.2
Protein Gi Number:
32483369
Ncbi Gene Id Number:
23595
Background Reference 1:
Pinto S, Quintana DG, Smith P, Mihalek RM, Hou ZH, Boynton S, Jones CJ, Hendricks M, Velinzon K, Wohlschlegel JA, Austin RJ, Lane WS, Tully T, Dutta A. latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant. Neuron 1999 May;23(1):45-54

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-101

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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