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Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ODC-1 antibody (SPM565)
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ODC-1 antibody (SPM565)

Ornithine Decarboxylase Antibody [SPM565]

CATALOG NUMBER: 34-148

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Western blot: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the Ornithine Decarboxylase antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
ODC1
Additional Names:
Ornithine decarboxylase, ODC, ODC1
Ncbi Gene Id Number:
4953
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

34-148

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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