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46-110 (0.2ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-110 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-110 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-110 (0.2ug/ml) staining of Human Spleen lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ORP5 Antibody

CATALOG NUMBER: 46-110

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: KEEAFLRRRFSLC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Spleen lysates (calculated MW of 98.4kDa according to NP_065947.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney and Liver. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
OSBPL5
Additional Names:
OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5
Protein Accession Number:
NP_065947.1,NP_663613.1,NP_001137535.1
Protein Gi Number:
14149740
Ncbi Gene Id Number:
114879
Background Reference 1:
Higashimoto K, Soejima H, Yatsuki H, Joh K, Uchiyama M, Obata Y, Ono R, Wang Y, Xin Z, Zhu X, Masuko S, Ishino F, Hatada I, Jinno Y, Iwasaka T, Katsuki T, Mukai T. Characterization and Imprinting Status of OBPH1/Obph1 Gene: Implications for an Extended Imprinting Domain in Human and Mouse. Genomics. 2002 Dec;80(6):575-84.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-110

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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