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Western blot analysis of extracts of various cell lines, using OSBPL3 antibody (19-424) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using OSBPL3 antibody (19-424) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 1s.

OSBPL3 Antibody

CATALOG NUMBER: 19-424

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 370-520 of human OSBPL3 (NP_056365.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
HeLa
Positive Control 3:
HepG2
Positive Control 4:
DU145
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 101kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
OSBPL3
Additional Names:
OSBPL3, ORP3, ORP-3, OSBP3, KIAA0704, MGC21526, oxysterol binding protein-like 3, OSBP-related protein 3, oxysterol-binding protein 3, oxysterol-binding protein-related protein 3
Ncbi Gene Id Number:
26031
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. The encoded protein is involved in the regulation of cell adhesion and organization of the actin cytoskeleton. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-424

List Size:
100 uL

List Price:

$436.00

Datasheet
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