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43-190 (1ug/ml) staining of U937 (A) and (0.3ug/ml) A431 (B) and HepG2 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-190 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
43-190 (1ug/ml) staining of U937 (A) and (0.3ug/ml) A431 (B) and HepG2 (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OSMR Antibody

CATALOG NUMBER: 43-190

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QIHGEQLDPHVT
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in lysates of cell line U937 and approx 70+125kDa in lysates of cell line A431 and HepG2 (calculated MW of 111kDa according to NP_003990.1 (isoform 1) and NP_001310435.1 (isoform 3), and 66.0kDa according to NP_001310436.1 (isoform 4)). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Specificity:
The immunizing peptide represents part of the extracellular domain.
Predicted Molecular Weight:
Approx 125kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
OSMR
Additional Names:
OSMR, oncostatin M receptor, MGC150626, MGC150627, MGC75127, OSMRB
Protein Accession Number:
NP_003990.1,NP_001161827.1,NP_001310435.1,NP_001310436.1
Protein Gi Number:
4557040
Ncbi Gene Id Number:
9180
Background Reference 1:
Wheeler HE, Metter EJ, Tanaka T, Absher D, Higgins J, Zahn JM, Wilhelmy J, Davis RW, Singleton A, Myers RM, Ferrucci L, Kim SK, Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-190

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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