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45-152 (0.1ug/ml) staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-152 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-152 (0.1ug/ml) staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Osteopontin Antibody

CATALOG NUMBER: 45-152

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-DPKSKEEDKHLK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in lysates of cell line MOLT4. This band corresponds to the cleavage product from the 60kDa glycoprotein (calculated MW of 33.8kDa according to NP_000573.1). Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1206 - MOLT4 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
SPP1
Additional Names:
SPP1, HGNC:11255, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), BNSP, BSPI, ETA-1, MGC110940, OPN, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), secreted phosphoprotein 1, SPP1/CALPHA1 fusion, bone sialoprotein I, early T-lymphocyte activation 1, osteopontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
Protein Accession Number:
NP_001035147.1,NP_000573.1,NP_001035149.1
Protein Gi Number:
4759166
Ncbi Gene Id Number:
6696
Background Reference 1:
Kolb A, Kleeff J, Guweidhi A, Esposito I, Giese NA, Adwan H, Giese T, Buchler MW, Berger MR, Friess H. Osteopontin Influences the Invasiveness of Pancreatic Cancer Cells and is Increased in Neoplastic and Inflammatory Conditions. Cancer Biol Ther. 2005 Jul 5;4(7)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-152

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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