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The purity of rh SPP1 / OPN was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of rh SPP1 / OPN was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.

Osteopontin Recombinant Protein

CATALOG NUMBER: 96-569

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ile 17 - Asn 300
Fusion Tag:
His Tag
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
33 kDa
Purity:
>80% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
SPP1
Additional Names:
SPP1, BNSP, OPN, Uropontin, Nephropontin, Osteopontin, BSP-1, ETA-1, BSPI
Protein Accession Number:
AAH07016
Ncbi Gene Id Number:
6696
Background:
Osteopontin (OPN) is also known as Secreted phosphoprotein 1 (SPP1), Bone sialoprotein 1, Nephropontin, Urinary stone protein, Uropontin, BNSP, which belongs to the osteopontin family. OPN / SPP1 is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure. Full length OPN (OPN-FL) can be modified by thrombin cleavage, which exposes a cryptic sequence, SVVYGLR on the cleaved form of the protein known as OPN-R. Osteopontin / SPP-1 is biosynthesized by a variety of tissue types. OPN is the ligand for integrin alpha-V/beta-3. OPN / SPP1 binds tightly to hydroxyapatite and appears to form an integral part of the mineralized matrix. OPN / SPP1 probably important to cell-matrix interaction. OPN / SPP1 acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Background Reference 1:
Wang KX., et al., 2008, Cytokine Growth Factor Rev. 19 (5-6): 333–45.
Background Reference 2:
Laffón A., et al., 1991, J. Clin. Invest. 88 (2): 546–52.
Background Reference 3:
Christensen B., et al., 2005, Biochem. J. 390:285-292.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-569

List Size:
0.1 mg

List Price:

$432.00

Datasheet
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