Osteoprotegerin Antibody [Bony-1]

Application Note:

Osteoprotegerin antibody can be used to recognize human OPG by Flow Cytometry (1:1,000) and Immunoprecipitation (1:200). Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1 μg/mL of MAb to OPG (human) (Bony-1). After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by Flow Cytometry. This product is for research use only.