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Western blot analysis of extracts of various cell lines, using OTC antibody (23-932) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human stomach using OTC antibody (23-932) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using OTC antibody (23-932) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using OTC antibody (23-932) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using OTC antibody (23-932) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

OTC Antibody

CATALOG NUMBER: 23-932

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_000522.3).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:100
Positive Control 1:
Mouse liver
Positive Control 2:
Mouse small intestine
Positive Control 3:
Rat liver
Positive Control 4:
Rat small intestine
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
OTC
Additional Names:
OTC, OCTD
Ncbi Gene Id Number:
5009
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This nuclear gene encodes a mitochondrial matrix enzyme. Missense, nonsense, and frameshift mutations in this enzyme lead to ornithine transcarbamylase deficiency, which causes hyperammonemia. Since the gene for this enzyme maps close to that for Duchenne muscular dystrophy, it may play a role in that disease also.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-932

List Size:
100 uL

List Price:

$436.00

Datasheet
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