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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
OTU7B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

OTU7B Antibody

CATALOG NUMBER: 57-615

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This OTU7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 757-784 amino acids from the C-terminal region of human OTU7B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
93 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OTUD7B
Additional Names:
OTU domain-containing protein 7B, Cellular zinc finger anti-NF-kappa-B protein, Zinc finger A20 domain-containing protein 1, Zinc finger protein Cezanne, OTUD7B, ZA20D1
Protein Accession Number:
Q6GQQ9
Protein Gi Number:
51701318
Ncbi Gene Id Number:
56957
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OTU7B has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.
Background Reference 1:
Bremm, A., et al. Nat. Struct. Mol. Biol. 17(8):939-947(2010)
Background Reference 2:
Jin, Z., et al. Cell 137(4):721-735(2009)
Background Reference 3:
Enesa, K., et al. J. Biol. Chem. 283(27):18582-18590(2008)
Background Reference 4:
Bohgaki, M., et al. Biochim. Biophys. Acta 1783(5):826-837(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-615

List Size:
400 ul

List Price:

$495.00

Datasheet
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