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Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μg/mL.
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μg/mL.
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Trial Size

OTUD4 Antibody

CATALOG NUMBER: 5075

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4.
The immunogen is located within the last 50 amino acids of OTUD4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
Predicted: 115, 123 kDa
Observed: 125 kDa
Purification:
OTUD4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
OTUD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OTUD4
Additional Names:
OTUD4 Antibody: HIN1, DUBA6, HSHIN1, HIN1, KIAA1046, OTU domain-containing protein 4, HIV-1-induced protein HIN-1
Protein Accession Number:
NP_001096123
Protein Gi Number:
156630992
Ncbi Gene Id Number:
54726
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).
Background Reference 1:
Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5075

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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