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Western blot analysis in Y79 cell line lysates (35ug/lane).
OTX2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Y79 cell line lysates (35ug/lane).

OTX2 Antibody

CATALOG NUMBER: 55-983

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Xenopus
Immunogen:
This OTX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-289 amino acids from the C-terminal region of human OTX2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
OTX2
Additional Names:
Homeobox protein OTX2, Orthodenticle homolog 2, OTX2
Protein Accession Number:
P32243
Ncbi Gene Id Number:
5015
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the bicoid sub-family ofhomeodomain-containing transcription factors. The encoded proteinacts as a transcription factor and may play a role in brain andsensory organ development. A similar protein in mice is requiredfor proper forebrain development. Two transcript variants encodingdistinct isoforms have been identified for this gene. Otheralternative splice variants may exist, but their full lengthsequences have not been determined.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Masuda, T., et al. J. Biol. Chem. 285(35):26933-26944(2010)
Background Reference 3:
Gonzalez-Rodriguez, J., et al. BrOphthalmol 94(8):1100-1104(2010)
Background Reference 4:
Chung, C.Y., et al. Brain 133 (PT 7), 2022-2031 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-983

List Size:
400 ul

List Price:

$495.00

Datasheet
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