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46-119 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-119 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-119 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

OX1 Receptor Antibody

CATALOG NUMBER: 46-119

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-YNFLSGKFREQFK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognise both the human proteins OX1R and OX2R.
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
HCRTR1
Additional Names:
OX1R, OX2R, HCRTR1, hypocretin (orexin) receptor 1, hypocretin receptor 1, hypocretin receptor-1, orexin receptor 1, orexin receptor-1, HCRTR2, hypocretin (orexin) receptor 2, hypocretin receptor 2, hypocretin receptor-2, orexin receptor 2, orexin receptor-2
Protein Accession Number:
NP_001516.2,NP_001517.2
Protein Gi Number:
4557637
Ncbi Gene Id Number:
3061;3062
Background Reference 1:
Fukunaka Y, Shinkai T, Hwang R, Hori H, Utsunomiya K, Sakata S, Naoe Y, Shimizu K, Matsumoto C, Ohmori O, Nakamura J The orexin 1 receptor (HCRTR1) gene as a susceptibility gene contributing to polydipsia-hyponatremia in schizophrenia Neuromolecular Med. 2007;9(4):292-7

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-119

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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