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42-523 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear s
42-523 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
42-523 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear s

Oxt Antibody

CATALOG NUMBER: 42-523

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: DGCRTDPACDPES
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 22kDa band in Mouse Liver after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9kDa according to NP_035155.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Oxt
Additional Names:
Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535
Protein Accession Number:
NP_035155.1
Protein Gi Number:
6754960
Ncbi Gene Id Number:
18429(mouse);25504(rat);

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-523

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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