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46-122 (0.5ug/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-122 (0.5ug/ml) staining of lysates of cell lines Daudi (A) and Caco-2 (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

P114-RHO-GEF Antibody

CATALOG NUMBER: 46-122

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SAKEDASKEDVIFF
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ARHGEF18
Additional Names:
ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18
Protein Accession Number:
NP_056133.2,NP_001124427.1
Protein Gi Number:
41327769
Ncbi Gene Id Number:
23370
Background Reference 1:
Blomquist A, Schworer G, Schablowski H, Psoma A, Lehnen M, Jakobs KH, Rumenapp U. Identification and characterization of a novel Rho-specific guanine nucleotide exchange factor. Biochem J. 2000 Dec 1;352 Pt 2:319-25.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-122

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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