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43-343 (1ug/ml) staining of Human Gastrointestinal cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
43-343 (1ug/ml) staining of Human Gastrointestinal cancer lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

p15INK4b isoform 2 Antibody

CATALOG NUMBER: 43-343

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-RQGARERGARP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 12+14kDa bands observed in Human Gastrointestinal cancer lysates (calculated MW of 8.1kDa according to NP_511042.1). The observed 14kDa cannot represent isoform 1 and therefore it most likely represents another posttranslational modification of isoform 2. Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize isoform 2 (NP_511042.1) only.
Predicted Molecular Weight:
Approx 12 + 14kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
p15INK4b
Additional Names:
CDKN2B, cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4), CDK4I, INK4B, MTS2, P15, TP15, p15INK4b, CDK inhibitory protein, CDK4B inhibitor, MTS-2, cyclin-dependent kinase 4 inhibitor B, cyclin-dependent kinases 4 and 6 binding protein, multiple tumor suppressor 2, p14-INK4b, p14_CDK inhibitor, p14_INK4B, p15 CDK inhibitor, p15-INK4b, p15_INK4B
Protein Accession Number:
NP_004927.2,NP_511042.1
Protein Gi Number:
17981694
Ncbi Gene Id Number:
1030
Background Reference 1:
Geyer CR.Strategies to re-express epigenetically silenced p15(INK4b) and p21(WAF1) genes in acute myeloid leukemia.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-343

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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