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Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.
Immunohistochemical analysis of paraffin - embedded human lung adenocarcinoma showing nuclear location using P16 antibody with DAB staining.
Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.

P16 Antibody [5A8A4 / 3G8D12]

CATALOG NUMBER: 32-172

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Human P16 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CDKN2A
Additional Names:
ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A
Protein Accession Number:
NP_000068.1
Protein Gi Number:
4502749
Ncbi Gene Id Number:
1029
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
p16 (cyclin-dependent kinase inhibitor 2A, INK4a) is a tumor suppressor protein. It is a specific inhibitor of Cdk 4 / Cdk 6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16 INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16 INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16 INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia.
Background Reference 1:
Bai, F. et al. Mol. Cell. Biol.2003 23, 1269-1277.
Background Reference 2:
Lowe, S.W. and Sherr, C.J. Curr. Opin. Genet.2003 Dev.13, 77-83.
Background Reference 3:
Sherr, C.J. Nat. Rev. Mol. Cell Biol.2001 2, 731-737.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-172

List Size:
0.1 mL

List Price:

$432.00

Datasheet
Shipping Info

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