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IHC testing of human colon stained with p27Kip1 antibody.
IHC testing of FFPE prostate carcinoma with p27Kip1 antibody
Intracellular FACS testing of HeLa cells with Alexa Fluor 488 conjugated p27Kip1 antibody (green) and isotype control (gray).
IHC testing of human colon stained with p27Kip1 antibody.

p27Kip1 Antibody [SX53G8]

CATALOG NUMBER: 33-108

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
CDKN1B
Additional Names:
Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Ncbi Gene Id Number:
1027
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-108

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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