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Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).

p27Kip1 (phospho Thr187) Antibody

CATALOG NUMBER: 79-196

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
p27Kip1 (Phospho-Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.
Predicted Molecular Weight:
27 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDKN1B
Additional Names:
KIP1, MEN4, CDKN4, MEN1B, P27KIP1, MAPTL, MTBT1, TAU, CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1
Protein Accession Number:
NP_004055.1
Protein Gi Number:
4757962
Ncbi Gene Id Number:
1027
Background:
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.
Background Reference 1:
Eguchi H, et al. (2003) Cancer Res; 63 (15): 4739-46Le XF, et al. (2003) J Biol Chem;Connor MK, et al. (2003) ) Mol Biol Cell; 14 (1): 201-13Fujita N, et al. (2002) J Biol Chem; 277 (32): 28706-13Ishida N, et al. (2000) J Biol Chem; 275 (33): 25146-54

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-196

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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