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42-772 staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
42-772 (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-772 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-772 staining (0.3ug/ml) of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

P2RX7 Antibody

CATALOG NUMBER: 42-772

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: YETNKVTRIQSMNY-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
P2RX7
Additional Names:
P2RX7, purinergic receptor P2X, ligand-gated ion channel, 7, MGC20089, P2X7, ATP receptor, P2X purinoceptor 7, P2X7 receptor, P2Z receptor, purinergic receptor P2X7
Protein Accession Number:
NP_002553.2
Protein Gi Number:
29294631
Ncbi Gene Id Number:
5027

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-772

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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