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P2RY1 Antibody

CATALOG NUMBER: 46-123

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KDLDNSPLRRK
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 38kDa in lysates of cell lines HEK293 and A549 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1kDa according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
P2RY1
Additional Names:
P2RY1, purinergic receptor P2Y, G-protein coupled, 1, P2Y1, ATP recepto, P2 purinoceptor subtype Y1, P2Y purinoceptor 1, platelet ADP receptor, purinergic receptor P2Y1
Protein Accession Number:
NP_002554.1
Protein Gi Number:
4505557
Ncbi Gene Id Number:
5028
Background Reference 1:
Arthur JF, Shen Y, Mu FT, Leon C, Gachet C, Berndt MC, Andrews RK. Calmodulin interacts with the platelet ADP receptor P2Y1. Biochem J. 2006 Sep 15;398(3):339-43.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-123

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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