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Western blot analysis of P450R antibody in K562 cell line lysates (35ug/lane)
P450R Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of P450R antibody in K562 cell line lysates (35ug/lane)

P450R Antibody

CATALOG NUMBER: 62-295

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This P450R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 462-488 amino acids from the Central region of human P450R.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POR
Additional Names:
NADPH--cytochrome P450 reductase, CPR, P450R, POR, CYPOR
Protein Accession Number:
P16435
Protein Gi Number:
2851393
Ncbi Gene Id Number:
5447
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
P450R is an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in its gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome.
Background Reference 1:
Fukami,M., J. Clin. Endocrinol. Metab. 94 (5), 1723-1731 (2009)
Background Reference 2:
Mast,N., Arch. Biochem. Biophys. 483 (1), 81-89 (2009)
Background Reference 3:
Brenner,S., FEBS J. 275 (18), 4540-4557 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-295

List Size:
400 ul

List Price:

$495.00

Datasheet
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