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Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.

p47 phox (phospho Ser345) Antibody

CATALOG NUMBER: 79-920

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
p47 phox (phospho Ser345) antibody was raised against a peptide sequence around phosphorylation site of Serine 345 (P-Q-S (p)-P-G) derived from Human Nuclear p47 phox.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345.
Predicted Molecular Weight:
45 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NCF1
Additional Names:
NCF1, P47 phox, NCF-47K
Protein Accession Number:
NP_000256.4
Protein Gi Number:
115298672
Ncbi Gene Id Number:
653361
Background:
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Background Reference 1:
Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Background Reference 2:
Lomax K.J., Science 245:409-412(1989).
Background Reference 3:
Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-920

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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