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43-227 (0.03ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-227 (0.03ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

p47phox Antibody

CATALOG NUMBER: 43-227

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DEAEDPDPNYAGEP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 40kDa band observed in Mouse Thymus lysates (calculated MW of 44.7kDa according to NP_035006.3). Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Lung, Thymus and Spleen and U937 for this particular batch.
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
p47phox
Additional Names:
Ncf1, neutrophil cytosolic factor 1, NOXO2, Ncf-1, p47, p47phox, 47 kDa neutrophil oxidase factor, NADPH oxidase subunit (47 kD), NADPH oxidase subunit (47kDa), NCF-47K, OTTMUSP00000022112, neutrophil NADPH oxidase factor 1, neutrophil cytosol factor 1, p47-phox
Protein Accession Number:
NP_035006.3
Protein Gi Number:
115298672
Ncbi Gene Id Number:
653361
Background Reference 1:
Roos D, Kuhns DB, Maddalena A, Bustamante J, Kannengiesser C, de Boer M, van Leeuwen K, Köker MY, Wolach B, Roesler J, Malech HL, Holland SM, Gallin JI, Stasia MJ, Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (second update).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-227

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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