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Western blot analysis in Ramos cell line lysates (35ug/lane).
Western blot analysis in Ramos cell line lysates (35ug/lane).

P4HA1 Antibody

CATALOG NUMBER: 58-934

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
61 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
P4HA1
Additional Names:
Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA
Protein Accession Number:
P13674
Protein Gi Number:
2507090
Ncbi Gene Id Number:
5033
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a component of prolyl 4-hydroxylase, akey enzyme in collagen synthesis composed of two identical alphasubunits and two beta subunits. The encoded protein is one ofseveral different types of alpha subunits and provides the majorpart of the catalytic site of the active enzyme. In collagen andrelated proteins, prolyl 4-hydroxylase catalyzes the formation of4-hydroxyproline that is essential to the proper three-dimensionalfolding of newly synthesized procollagen chains. Alternativelyspliced transcript variants encoding different isoforms have beendescribed.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Gorres, K.L., et al. PLoS ONE(11), E7635 (2009) :
Background Reference 3:
Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
Background Reference 4:
Fahling, M., et al. J. Biol. Chem. 281(36):26089-26101(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-934

List Size:
400 ul

List Price:

$495.00

Datasheet
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