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Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific). Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in 293 cell line lysates (35ug/lane).
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

p53 Antibody

CATALOG NUMBER: 61-402

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:100
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TP53
Additional Names:
Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53
Protein Accession Number:
P04637
Protein Gi Number:
269849759
Ncbi Gene Id Number:
7157
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.
Background Reference 1:
Chen L, et al. Oncogene. 2003 Aug 14;22(34):5348-57.
Background Reference 2:
Melchior F, et al. Cell Cycle. 2002 Jul-Aug;1(4):245-9.
Background Reference 3:
Kahyo T, et al. Mol Cell. 2001 Sep;8(3):713-8.
Background Reference 4:
Kwek SS, et al. Oncogene. 2001 May 3;20(20):2587-99.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-402

List Size:
400 ul

List Price:

$495.00

Datasheet
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