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Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p53 (Phospho-Ser15).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser15).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p53 (Phospho-Ser15).
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p53 (Phospho-Ser15).

p53 (phospho Ser15) Antibody

CATALOG NUMBER: 79-146

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
p53 (Phospho-Ser15) antibody was raised against a peptide sequence around phosphorylation site of serine 15 (P-L-S (p) -Q-E) derived from Human p53.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of p53 only whenphosphorylated at serine15.
Predicted Molecular Weight:
53 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
TP53
Additional Names:
P53, BCC7, LFS1, TRP53, MEK6, MKK6, PRKMK6, SKK3, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53
Protein Accession Number:
NP_000537.3
Protein Gi Number:
120407068
Ncbi Gene Id Number:
7157
Background:
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.
Background Reference 1:
Lin T, et al. (2005) Nat Cell Biol; 7 (2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23): 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38): 39584-92.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-146

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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