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Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.
Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

p53R2 Antibody

CATALOG NUMBER: 2383

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
p53R2 antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2.
The immunogen is located within the first 50 amino acids of p53R2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
p53R2 antibody can be used for detection of p53R2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 3:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 4:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 39 kDa
Observed: 43 kDa
Purification:
p53R2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
p53R2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
p53R2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RRM2B
Additional Names:
p53R2 Antibody: P53R2, MTDPS8A, MTDPS8B, P53R2, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53R2
Protein Accession Number:
BAA92434
Protein Gi Number:
7229086
Ncbi Gene Id Number:
50484
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
p53R2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumor suppression.
Background Reference 1:
Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404:42-9.
Background Reference 2:
Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998;2 82:1893-7.
Background Reference 3:
Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
Background Reference 4:
Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 2000; 102:849-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2383

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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