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Western blot analysis of lysed extracts from JK cells using p56Dok-2 (Ab-299).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2 (Ab-299).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using p56Dok-2 (Ab-299).
Western blot analysis of lysed extracts from JK cells using p56Dok-2 (Ab-299).

p56Dok 2 (Ab 299) Antibody

CATALOG NUMBER: 79-394

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
p56Dok-2 (Ab-299) antibody was raised against a peptide sequence around aa.297~301 (G-E-Y-A-V) derived from Human p56Dok-2.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total p56Dok-2 protein.
Predicted Molecular Weight:
56 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
DOK2
Additional Names:
p56DOK, p56dok-2, CHK1, DOK2
Protein Accession Number:
NP_003965.2
Protein Gi Number:
41406050
Ncbi Gene Id Number:
9046
Background:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation
Background Reference 1:
Feng Cong, et, al. (1999) Mol. Cell. Biol; 19: 8314 - 8325. Serge Lemay, et, al. (2000) Mol. Cell. Biol; 20: 2743 - 2754. Ute Schaeper, et, al. (2000) J. Cell Biol; 149: 1419. Miyuki Honma, et, al. (2006) Genes Cells; 11: 143 - 151.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-394

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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