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Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

P5F1B Antibody

CATALOG NUMBER: 55-446

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig
Immunogen:
This P5F1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the C-terminal region of human P5F1B.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
POU5F1B
Additional Names:
Putative POU domain, class 5, transcription factor 1B, Oct4-pg1, Octamer-binding protein 3-like, Octamer-binding transcription factor 3-like, POU5F1B
Protein Accession Number:
Q06416
Protein Gi Number:
357528826
Ncbi Gene Id Number:
5462
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This intronless gene was thought to be a transcribedpseudogene of POU class 5 homeobox 1, however, it has been reportedthat this gene can encode a functional protein. The encoded proteinis nearly the same length as and highly similar to the POU class 5homeobox 1 transcription factor, has been shown to be a weaktranscriptional activator and may play a role in carcinogenesis andeye development.
Background Reference 1:
Kastler, S., et al. Prostate 70(6):666-674(2010)
Background Reference 2:
White, K.L., et al. Twin Res Hum Genet 13(1):43-56(2010)
Background Reference 3:
Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010)
Background Reference 4:
Gudmundsson, J., et al. Nat. Genet. 41(10):1122-1126(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-446

List Size:
400 ul

List Price:

$495.00

Datasheet
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