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Western blot analysis of PAAF1 Antibody in A549 cell line lysates (35ug/lane)
PAAF1 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of PAAF1 Antibody in A549 cell line lysates (35ug/lane)

PAAF1 Antibody

CATALOG NUMBER: 61-776

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PAAF1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PAAF1
Additional Names:
Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71, PAAF1, WDR71
Protein Accession Number:
Q9BRP4
Protein Gi Number:
97217547
Ncbi Gene Id Number:
80227
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PAAF1 inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.
Background Reference 1:
Lassot, I., et al. Mol. Cell 25(3):369-383(2007)
Background Reference 2:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Background Reference 3:
Ponnappan, S., et al. Int. J. Biochem. Cell Biol. 39(4):799-809(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-776

List Size:
400 ul

List Price:

$495.00

Datasheet
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