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Western blot analysis of extracts of various cell lines, using PABPC1 antibody (15-770) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of L929 cells using PABPC1 Polyclonal Antibody (15-770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using PABPC1 Polyclonal Antibody (15-770) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PABPC1 antibody (15-770) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

PABPC1 Antibody

CATALOG NUMBER: 15-770

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
A-549
Positive Control 2:
HT-29
Positive Control 3:
HeLa
Positive Control 4:
Mouse brain
Positive Control 5:
Rat lung
Predicted Molecular Weight:
Observed: 71kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PABPC1
Additional Names:
PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1
Ncbi Gene Id Number:
26986
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-770

List Size:
100 uL

List Price:

$436.00

Datasheet
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