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Western blot analysis of extracts of various cell lines, using PABPC5 antibody (24-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using PABPC5 antibody (24-022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

PABPC5 Antibody

CATALOG NUMBER: 24-022

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-382 of human PABPC5 (NP_543022.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:4000
IHC: 1:50 - 1:200
Positive Control 1:
SH-SY5Y
Positive Control 2:
A375
Positive Control 3:
MCF7
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 43kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PABPC5
Additional Names:
PABP5, polyadenylate-binding protein 5
Ncbi Gene Id Number:
140886
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that binds to the polyA tail found at the 3' end of most eukaryotic mRNAs. It is thought to play a role in the regulation of mRNA metabolic processes in the cytoplasm. This gene is located in a gene-poor region within the X-specific 13d-sY43 subinterval of the chromosome Xq21.3/Yp11.2 homology block. It is located close to translocation breakpoints associated with premature ovarian failure, and is therefore a potential candidate gene for this disorder.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-022

List Size:
100 uL

List Price:

$436.00

Datasheet
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