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46-742 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-742 (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-742 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PACE4 Antibody

CATALOG NUMBER: 46-742

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: PDCEPGTYFDSE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx. 70kDa band in Human, Mouse and Rat Liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 105 to 107kDa according to NP_002561.1, NP_612192.1, NP_612193.1 and NP_612194.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis and Skeletal Muscle. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize reported isoforms a, b, g, h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PCSK6
Additional Names:
PCSK6, proprotein convertase subtilisin/kexin type 6, PACE4, SPC4, paired basic amino acid cleaving system 4, subtilisin-like proprotein convertase 4, subtilisin-like protease, subtilisin/kexin-like protease PACE4
Protein Accession Number:
NP_002561.1,NP_612192.1,NP_612195.1,NP_612197.1,NP_612196.1,NP_612198.2,NP_612193.1,NP_612194.1
Protein Gi Number:
4505577
Ncbi Gene Id Number:
5046
Background Reference 1:
Jin W, Wang X, Millar JS, Quertermous T, Rothblat GH, Glick JM, Rader DJ. Hepatic proprotein convertases modulate HDL metabolism. Cell Metab. 2007 Aug;6(2):129-36.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-742

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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