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Western blot analysis of PACS2 in mouse brain tissue lysate with PACS2 antibody at 1 μg/ml.
Immunohistochemistry of PACS2 in mouse brain tissue with PACS2 antibody at 5 μg/mL.
Immunofluorescence of PACS2 in mouse brain tissue with PACS2 antibody at 20 μg/ml.<br><br>Red: PACS2 Antibody (8189) <br> Blue: DAPI staining
Western blot analysis of PACS2 in mouse brain tissue lysate with PACS2 antibody at 1 μg/ml.
Trial Size

PACS2 Antibody

CATALOG NUMBER: 8189

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PACS2 antibody was raised against a 17 amino acid peptide near the center of human PACS2.
The immunogen is located within amino acids 330 - 380 of PACS2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PACS2 antibody can be used for detection of PACS2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
PACS2 antibody is human, mouse and rat reactive. At least three isoforms of PACS2 are known to exist; this antibody will detect all three. PACS2 antibody is predicted to not cross-react with PACS1.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 98 kDa
Observed: 95 kDa
Purification:
PACS2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PACS2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PACS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PACS2
Additional Names:
Phosphofurin acidic cluster sorting protein 2, PACS-2, PACS1-like protein, PACS1L
Protein Accession Number:
NP_056012
Protein Gi Number:
155029546
Ncbi Gene Id Number:
23241
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PACS2 (phosphofurin acidic cluster sorting protein-2), PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS family (1,2). It is expressed in a broad range of tissues with highest expression in skeletal muscle, brain, heart, testis and pancreas (2,). PACS2 interacts with Polycystin-2 and BID and functions as a sorting protein that regulates mitochondria-ER communication and is thought to be involved in ion channel trafficking, specifically direct cluster-containing ion channels to distinct subcellular compartments (3-5).
Background Reference 1:
Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
Background Reference 2:
Brasacchio D, Noori T, House C, et al. A functional genomics screen identifies PCAF and ADA3 as regulators of human granzyme B-mediated apoptosis and Bid cleavage. Cell Death Differ. 2014; 21:748-60.
Background Reference 3:
Werneburg NW, Bronk SF, Guicciardi ME, et al. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein-induced lysosomal translocation of proapoptotic effectors is mediated by phosphofurin acidic cluster sorting protein-2 (PACS-2). J. Biol. Chem. 2012; 287:24427-37.
Background Reference 4:
You H and Thomas G. A homeostatic switch in PACS-2 links membrane traffic to TRAIL-induced apoptosis. Cell Cycle 2009; 8:2679-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8189

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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